<sup>1</sup>H, <sup>13</sup>C and <sup>15</sup>N backbone resonance assignment of the RNA binding motif 39 (RBM39) tandem RNA recognition motifs (RRM1-2).
Unraveling the RNA-binding secrets of RBM39: NMR reveals the intricate backbone of its tandem RRM domains, paving the way for understanding its role in splicing.
This study utilized NMR spectroscopy to assign the backbone resonances of the tandem RNA recognition motifs (RRM1-2) of the human splicing factor RBM39. The researchers successfully obtained a near-complete assignment of the 1H, 15N, and 13C resonances, which allowed them to predict the secondary structure of the RRM1-2 domains. The predicted structures closely matched the known solution structures of the individual RRM domains, as well as the AlphaFold2 model, validating the reliability of the NMR data. This comprehensive characterization of the RBM39 RRM1-2 backbone provides a valuable foundation for further investigations into the structural basis of its RNA-binding and splicing functions.